A modified method for enzymatic isolation of and subsequent wax extraction from Arabidopsis thaliana leaf cuticle
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F20%3AA210266L" target="_blank" >RIV/61988987:17310/20:A210266L - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/20:00533883 RIV/60076658:12310/20:43901407 RIV/61989100:27710/20:10245487
Result on the web
<a href="https://rdcu.be/b7wyi" target="_blank" >https://rdcu.be/b7wyi</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13007-020-00673-7" target="_blank" >10.1186/s13007-020-00673-7</a>
Alternative languages
Result language
angličtina
Original language name
A modified method for enzymatic isolation of and subsequent wax extraction from Arabidopsis thaliana leaf cuticle
Original language description
Background The plant cuticle represents one of the major adaptations of vascular plants to terrestrial life. Cuticular permeability and chemical composition differ among species. Arabidopsis thaliana is a widely used model for biochemical and molecular genetic studies in plants. However, attempts to isolate the intact cuticle from fresh leaves of Arabidopsis have failed so far. The goal of this study was to optimise an enzymatic method for cuticle isolation of species with a thin cuticle and to test it on several A. thaliana wild types and mutants. Results We developed a method for isolation of thin cuticles that allows reducing the isolation time, the separation of abaxial and adaxial cuticles, and avoids formation of wrinkles. Optical microscopy was used for studying cuticle intactness and scanning electron microscopy for visualisation of external and internal cuticle structures after isolation. Wax extracts were analysed by GC–MS. Isolation of intact cuticle was successful for all tested plants. The wax compositions (very-long-chained fatty acids, alcohols and alkanes) of intact leaves and isolated cuticles of wild type Col-0 were compared. Conclusions We conclude that the optimised enzymatic method is suitable for the isolation of A. thaliana adaxial and abaxial cuticles. The isolated cuticles are suitable for microscopic observation. Analysis of wax composition revealed some discrepancies between isolated cuticles and intact leaves with a higher yield of wax in isolated cuticles.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Methods
ISSN
1746-4811
e-ISSN
1746-4811
Volume of the periodical
—
Issue of the periodical within the volume
16
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
129
UT code for WoS article
000574284700002
EID of the result in the Scopus database
2-s2.0-85092480187