Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202AFN" target="_blank" >RIV/61988987:17310/21:A2202AFN - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/21:00553251 RIV/00216208:11310/21:10433805
Result on the web
<a href="https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-021-01078-1" target="_blank" >https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-021-01078-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12915-021-01078-1" target="_blank" >10.1186/s12915-021-01078-1</a>
Alternative languages
Result language
angličtina
Original language name
Genomics and transcriptomics yields a system-level view of the biology of the pathogen Naegleria fowleri
Original language description
Background The opportunistic pathogen Naegleria fowleri establishes infection in the human brain, killing almost invariably within 2 weeks. The amoeba performs piece-meal ingestion, or trogocytosis, of brain material causing direct tissue damage and massive inflammation. The cellular basis distinguishing N. fowleri from other Naegleria species, which are all non-pathogenic, is not known. Yet, with the geographic range of N. fowleri advancing, potentially due to climate change, understanding how this pathogen invades and kills is both important and timely. Results Here, we report an -omics approach to understanding N. fowleri biology and infection at the system level. We sequenced two new strains of N. fowleri and performed a transcriptomic analysis of low- versus high-pathogenicity N. fowleri cultured in a mouse infection model. Comparative analysis provides an in-depth assessment of encoded protein complement between strains, finding high conservation. Molecular evolutionary analyses of multiple diverse cellular systems demonstrate that the N. fowleri genome encodes a similarly complete cellular repertoire to that found in free-living N. gruberi. From transcriptomics, neither stress responses nor traits conferred from lateral gene transfer are suggested as critical for pathogenicity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC BIOLOGY
ISSN
1741-7007
e-ISSN
1741-7007
Volume of the periodical
19
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
18
Pages from-to
142
UT code for WoS article
000680417900001
EID of the result in the Scopus database
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