The larval gut of Spodoptera frugiperda harbours culturable bacteria with metabolic versatility after insecticide exposure

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F25%3AA2603A5G" target="_blank" >RIV/61988987:17310/25:A2603A5G - isvavai.cz</a>

Result on the web

<a href="https://resjournals.onlinelibrary.wiley.com/doi/full/10.1111/imb.12983?af=R" target="_blank" >https://resjournals.onlinelibrary.wiley.com/doi/full/10.1111/imb.12983?af=R</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/imb.12983" target="_blank" >10.1111/imb.12983</a>

Result language

angličtina

Original language name

The larval gut of Spodoptera frugiperda harbours culturable bacteria with metabolic versatility after insecticide exposure

Original language description

Spodoptera frugiperda (fall armyworm) poses a substantial risk to crops worldwide, resulting in considerable economic damage. The gut microbiota of insects plays crucial roles in digestion, nutrition, immunity, growth and, sometimes, the degradation of insecticides. The current study examines the effect of synthetic insecticides on the gut microbiomeof third instar S. frugiperda larvae using both culture-dependent techniques and 16SrRNA gene sequencing for bacterial community profiling and diversity analysis. Inuntreated larvae, the sequencing approach revealed a diverse microbiome dominated bythe phyla Firmicutes, Proteobacteria and Bacteroidota, with key genera including Bacteroides,Faecalibacterium and Pelomonas. In parallel, 323 bacterial strains were isolated and assigned to the orders Bacillales, Burkholderiales, Enterobacterales, Flavobacteriales,Lactobacillales, Micrococcales, Neisseriaies, Pseudomonadales, Sphingobacteriales and Xanthomonadales. The prevailing culturable species included Serratia marcescens, Klebsiella variicola and Enterobacter quasiroggenkampii. Treatment with sublethal concentrations of three insecticides (broflanilide, spinosad and indoxacarb) caused significant changes in gut microbiome diversity and composition. Treated larvae showed a shift towards increased Proteobacteria abundance and decreased Firmicutes. Specifically, Acinetobacter and Rhodococcus were dominant in treated samples. Functional predictions highlighted significant metabolic versatility involving nutrient processing, immune response, detoxification, xenobiotic metabolism, and stress response, suggesting microbialadaptation to insecticide exposure. Network correlation analysis highlighted disrupted microbial interactions and altered community structures under insecticide treatment. These findings enhance our understanding of how insecticides impact the gut microbiota in S. frugiperda and may inform future strategies for managing pest resistance through microbiome-based approaches.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10616 - Entomology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Insect Molecular Biology

ISSN

0962-1075

e-ISSN

1365-2583

Volume of the periodical

—

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

452-469

UT code for WoS article

001420886500001

EID of the result in the Scopus database

2-s2.0-85219684200