Lipid Phase Behaviour of the Curvature Region of Thylakoid Membranes of <scp><i>Spinacia oleracea</i></scp>
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F25%3AA2603B4B" target="_blank" >RIV/61988987:17310/25:A2603B4B - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1111/ppl.70289" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/ppl.70289</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppl.70289" target="_blank" >10.1111/ppl.70289</a>
Alternative languages
Result language
angličtina
Original language name
Lipid Phase Behaviour of the Curvature Region of Thylakoid Membranes of <scp><i>Spinacia oleracea</i></scp>
Original language description
Thylakoid membranes (TMs) of oxygenic photosynthetic organisms are flat membrane vesicles, which form highly organised, interconnected membrane networks. In vascular plants, they are differentiated into stacked and unstacked regions, the grana and stroma lamellae, respectively; they are densely packed with protein complexes performing the light reactions of photosynthesis and generating a proton motive force (pmf). The maintenance of pmf and its utilisation for ATP synthesis requires sealing the TMs at their highly curved regions (CRs). These regions are devoid of chlorophyll‐containing proteins but contain the curvature‐inducing CURVATURE THYLAKOID1 (CURT1) proteins and are enriched in lipids. Because of the highly curved nature of this region, at the margins of grana and stroma TMs, the molecular organisation of lipid molecules is likely to possess distinct features compared to those in the major TM domains. To clarify this question, we isolated CR fractions from Spinacia oleracea</jats:italic></jats:styled-content> and, using BN‐PAGE and western blot analysis, verified that they are enriched in CURT1 proteins and in lipids. The lipid phase behaviour of these fractions was fingerprinted with P‐NMR spectroscopy, which revealed that the bulk lipid molecules assume a non‐bilayer, isotropic lipid phase. This finding underpins the importance of the main, non‐bilayer lipid species, monogalactosyldiacylglycerol, of TMs in their self‐assembly and functional activity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
<a href="/en/project/GA23-07744S" target="_blank" >GA23-07744S: Identification and characterization of structural entities associated with different lipid phases of plant thylakoid membranes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiologia Plantarum
ISSN
0031-9317
e-ISSN
1399-3054
Volume of the periodical
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Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
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UT code for WoS article
001509967400001
EID of the result in the Scopus database
2-s2.0-105008235513