Restriction Site Detection in Optical Mapping Data

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27240%2F22%3A10252093" target="_blank" >RIV/61989100:27240/22:10252093 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/chapter/10.1007/978-3-031-14627-5_40" target="_blank" >https://link.springer.com/chapter/10.1007/978-3-031-14627-5_40</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-3-031-14627-5_40" target="_blank" >10.1007/978-3-031-14627-5_40</a>

Result language
angličtina
Original language name
Restriction Site Detection in Optical Mapping Data
Original language description
Optical Mapping is a method of DNA sequencing that is used to detect large structural variations in genomes. To create these optical maps a restriction enzyme is mixed with DNA. The enzyme binds to DNA and creates labels called restriction sites. These labels are captured by a camera where they appear as bright spots. This work introduces two methods to find these high-intensity points in optical maps. The first method is trying to find the peaks based only on intensity levels and the second is using a signal-to-noise ratio. Both methods have more than three parameters that can affect the results. Differential evolution and particle swarm optimization were used to find the best parameters that would give the highest accuracy. Bionano results were used as ground truth.
Czech name
—
Czech description
—

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Lecture Notes in Networks and Systems. Volume 527
ISBN
978-3-031-14626-8
ISSN
2367-3370
e-ISSN
2367-3389
Number of pages
10
Pages from-to
393-402
Publisher name
Springer
Place of publication
Cham
Event location
Sanda
Event date
Sep 7, 2022
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000870692600040

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