Methods for analysis of human amniotic fluids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27640%2F14%3A86091453" target="_blank" >RIV/61989100:27640/14:86091453 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Methods for analysis of human amniotic fluids

Original language description

Analysis of bodyfluids is new interesting approach for researching air-pollution impact on human health. Bodyfluids make an internal environment of tissues and organs and therefore bodyfluids composition can affect their functions. Bodyfluids are in touch with outer environment mostly through the respiratory tract, and thus inhalation of polluted air may lead to penetration of some chemicals into the bodyfluids. Air-polluting suspended solid particles are able to penetrate biomembranes due to their size below 100nm. One of these biomembranes is placenta, which prevents from penetrating harmful substances to amniotic fluid. Amniotic fluid provides fetus protection and fetus nutrition from mother's blood. Therefore, its composition is crucial for fetus development. Collected samples of human amniotic fluid were analyzed using the selected techniques: Raman microspectroscopy, Scanning electron microscopy with energy-dispersed spectrometer (SEM-EDS), Fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. Major natural compounds in human amniotic fluid are sodium chloride and organic compounds, mainly proteins, that were analyzed by XRD, SEM-EDS, FTIR and Raman microspectroscopy. In collected samples were also found metal-based particles. These results were obtained by SEM-EDS and Raman microspectroscopy. SEM-EDS analysis seems to be most suitable, because it is quite fast and precise data obtaining. Compared to Raman microspectroscopy, which is more time-consuming method. However, Raman microspectroscopy is a valuable tool for point-phase-analysis. XRD wasn't able to detect metal and its compounds presence, because of its detection limit.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

DN - Environmental impact on health

OECD FORD branch

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Project

<a href="/en/project/EE.2.3.20.0074" target="_blank" >EE.2.3.20.0074: Nanotechnology - the basis for international cooperation</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON 2013 : 5th international conference : October 16th-18th 2013, hotel Voroněž I, Brno, Czech Republic, EU : conference proceedings [CD-ROM]

ISBN

978-80-87294-47-5

ISSN

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e-ISSN

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Number of pages

6

Pages from-to

681-686

Publisher name

Tanger

Place of publication

Ostrava

Event location

Brno

Event date

Oct 16, 2013

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000352070900121