Multiscale imaging of plant development by light-sheet fluorescence microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27740%2F18%3A10240516" target="_blank" >RIV/61989100:27740/18:10240516 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/18:73590033
Result on the web
<a href="https://www.nature.com/articles/s41477-018-0238-2" target="_blank" >https://www.nature.com/articles/s41477-018-0238-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41477-018-0238-2" target="_blank" >10.1038/s41477-018-0238-2</a>
Alternative languages
Result language
angličtina
Original language name
Multiscale imaging of plant development by light-sheet fluorescence microscopy
Original language description
Light-sheet fluorescence microscopy (LSFM) methods collectively represent the major breakthrough in developmental bio-imaging of living multicellular organisms. They are becoming a mainstream approach through the development of both commercial and custom-made LSFM platforms that are adjusted to diverse biological applications. Based on high-speed acquisition rates under conditions of low light exposure and minimal photo-damage of the biological sample, these methods provide ideal means for long-term and in-depth data acquisition during organ imaging at single-cell resolution. The introduction of LSFM methods into biology extended our understanding of pattern formation and developmental progress of multicellular organisms from embryogenesis to adult body. Moreover, LSFM imaging allowed the dynamic visualization of biological processes under almost natural conditions. Here, we review the most important, recent biological applications of LSFM methods in developmental studies of established and emerging plant model species, together with up-to-date methods of data editing and evaluation for modelling of complex biological processes. Recent applications in animal models push LSFM into the forefront of current bio-imaging approaches. Since LSFM is now the single most effective method for fast imaging of multicellular organisms, allowing quantitative analyses of their long-term development, its broader use in plant developmental biology will likely bring new insights.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Plants
ISSN
2055-026X
e-ISSN
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Volume of the periodical
4
Issue of the periodical within the volume
9
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
"639–650"
UT code for WoS article
000446639100013
EID of the result in the Scopus database
2-s2.0-85052996177