Imaging plant cells and organs with light-sheet and super-resolution microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F22%3A73613960" target="_blank" >RIV/61989592:15640/22:73613960 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/22:73613960
Result on the web
<a href="https://academic.oup.com/plphys/article/188/2/683/6358151" target="_blank" >https://academic.oup.com/plphys/article/188/2/683/6358151</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/plphys/kiab349" target="_blank" >10.1093/plphys/kiab349</a>
Alternative languages
Result language
angličtina
Original language name
Imaging plant cells and organs with light-sheet and super-resolution microscopy
Original language description
The documentation of plant growth and development requires integrative and scalable approaches to investigate and spatiotemporally resolve various dynamic processes at different levels of plant body organization. The present update deals with vigorous developments in mesoscopy, microscopy and nanoscopy methods that have been translated to imaging of plant subcellular compartments, cells, tissues and organs over the past 3 years with the aim to report recent applications and reasonable expectations from current light-sheet fluorescence microscopy (LSFM) and super-resolution microscopy (SRM) modalities. Moreover, the shortcomings and limitations of existing LSFM and SRM are discussed, particularly for their ability to accommodate plant samples and regarding their documentation potential considering spherical aberrations or temporal restrictions prohibiting the dynamic recording of fast cellular processes at the three dimensions. For a more comprehensive description, advances in living or fixed sample preparation methods are also included, supported by an overview of developments in labeling strategies successfully applied in plants. These strategies are practically documented by current applications employing model plant Arabidopsis thaliana (L.) Heynh., but also robust crop species such as Medicago sativa L. and Hordeum vulgare L. Over the past few years, the trend towards designing of integrative microscopic modalities has become apparent and it is expected that in the near future LSFM and SRM will be bridged to achieve broader multiscale plant imaging with a single platform.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLANT PHYSIOLOGY
ISSN
0032-0889
e-ISSN
1532-2548
Volume of the periodical
188
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
20
Pages from-to
683-702
UT code for WoS article
000761476800003
EID of the result in the Scopus database
2-s2.0-85124658565