Molecular-biological analysis of vancomycin-resistant enterococci isolated from a community in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F04%3A00000892" target="_blank" >RIV/61989592:15110/04:00000892 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Molecular-biological analysis of vancomycin-resistant enterococci isolated from a community in the Czech Republic

Original language description

Objective: To determine the occurence of vancomycin-resistant enterococci (VRE) in a community of the Czech Republic and a molecular-biological analysis of the VRE isolated. Methods: Enterococci were isolated from the rectal swabs of healthy people in the Olomouc region (population 300,000), Czech Republic in the period of Januar - December 2003. The molecular-biological analysis of VRE was performed by analysis of isolated DNA, which was cleaved by restriction enzyme Smal and separated by pulse field gel electrophoresis (PFGE). Results: A total number of 5,283 swabs were evaluated and 558 Enterococcus sp. strains were isolated during the follow-up period. 9 strains (1.6%) were identified as VRE. Two strains were E. faecium phenotype VanA, one strain was E. faecalis phenotype VanB, two strains were E. gallinarum phenotype VanC and four strains were E. casseliflavus phenotype VanC. PFGE was used to obtain 9 different restriction profiles of VRE strains. The analysis showed closer a sim

Czech name

Molekulárně-biologická analýza vankomycin-rezistentních enterokoků izolovaných z komunitního prostředí České republiky

Czech description

Enterokoky se staly závažným problémem vzhledem ke své etiologické roli v bakteriálních infekcích. Zvyšuje se významně podíl enterokoků s rezistencí k vankomycinu a z tohoto důvodu je šíření těchto kmenů velmi důležitou epidemiologickou problematikou. Cílem této studie bylo monitorovat enterokoky s rezistencí k vankomycinu v ČR a provést jejich molekulárně biologickou analýzu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/NI7305" target="_blank" >NI7305: Sources, spreading and possibilites of prevention of vancomycin-resistant enterococci</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Volume of the periodical

2

Issue of the periodical within the volume

148

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

3

Pages from-to

167-169

UT code for WoS article

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EID of the result in the Scopus database

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