Cyclosporin A potentiates the cytotoxic effects of methyl methanesulphonate in HL-60 and K562 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F07%3A00003559" target="_blank" >RIV/61989592:15110/07:00003559 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cyclosporin A potentiates the cytotoxic effects of methyl methanesulphonate in HL-60 and K562 cells

Original language description

Methyl methanesulphonate (MMS) is a DNA damaging agent, which induces oxidative stress, ATP depletion, and consequently, cell death, in HL-60 and K562 cells. The cell death induced by MMS predominantly exhibited the morphological and biochemical hallmarks of necrosis. A minor population of dying cells exhibited apoptotic hallmarks, especially in K562 cell cultures. Cyclosporin A (CsA) was used to modulate the MMS-induced cell death. Our results indicated that CsA did not prevent cells from dying, but changed the mode of death from necrotic to apoptotic. Surprisingly, CsA enhanced oxidative stress and increased the overall number of dead cells. Based on these results, we conclude that the modulatory effect of CsA on MMS-induced cell death might arise from an interference by CsA with mitochondrial metabolism, rather than from inhibition of the MMS efflux mediated by P-glycoprotein.

Czech name

Cyklosporin A potenciuje cytotoxický účinek methyl methanesulfonátu u buněčných linií HL-60 a K562

Czech description

Methyl methanesulfonát (MMS) je látka, která u buněk HL-60 a K562 poškozuje DNA působením oxidačního stresu; dále způsobuje depleci ATP a v důsledku těchto působení vede k buněčné smrti. Buněčná smrt indukovaná působením MMS vykazovala morfologické a biochemické znaky nekrózy. Minoritní část umírajících buněk vykazovala známky apoptózy, zvláště u buněk linie K562. ...

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/GA301%2F04%2F1239" target="_blank" >GA301/04/1239: Sensitivity and resistance of Bcr/Abl leukemia cells to STI571 in vitro</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ATLA - Alternatives to Laboratory Animals

ISSN

0261-1929

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

79-85

UT code for WoS article

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EID of the result in the Scopus database

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