Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F08%3A00006992" target="_blank" >RIV/61989592:15110/08:00006992 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/08:00005343

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine

Original language description

The quaternary benzo[c]phenanthridine alkaloid chelerythrine is widely used as an inhibitor of protein kinase C (PKC). However, in biological systems chelerythrine interacts with an array of proteins. In this study, we examined the effects of chelerythrine and sanguinarine on conventional PKCs (cPKCs) and PKC upstream kinase, phosphoinositide-dependent protein kinase 1 (PDK1), under complete inhibition conditions of PKC-dependent oxidative burst. In neutrophil-like HL-60 cells, sanguinarine and chelerythrine inhibited N-formyl-Met-Leu-Phe, phorbol 12-myristate 13-acetate (PMA)-, and A23187-induced oxidative burst with IC(50) values not exceeding 4.6 mumol/L, but the inhibition of PMA-stimulated cPKC activity in intact cells required at least fivefold higher alkaloid concentrations. At concentrations below 10 mumol/L, sanguinarine and chelerythrine prevented phosphorylation of approximately 80 kDa protein and sequestered approximately 60 kDa phosphoprotein in cytosol.

Czech name

Isoenzymy konvenční protein kinasy C podléhají defosforylaci v neutrofilům podobných HL-60 buňkách inkubovaných s chelerythrinem a sanguinarinem.

Czech description

Kvartérní benzo[c]fenanthridinový alkaloid chelerythrin je široce využíván jako inhibitor protein kinasy C (PKC). V biologických systémech ale chelerythrin interaguje s plejádou proteinů. V této studii jsme zkoumali účinky chelerythrinu a sanguinarinu nakonvenční PKCs (cPKCs) a PKC upstream kinasu, fosfoinositidedependent protein kinasu 1 (PDK1), za podmínek kompletní inhibice PKC-dependentního oxidativního vzplanutí. V neutrofilům podobných buňkách HL-60 cells, inhibovaly sanguinarin a chelerythrin N-formyl-Met-Leu-Phe, phorbol 12-myristate 13-acetate (PMA)-, a A23187-indukované oxidativní vzplanutí, s hodnotami IC50 nepřesahující 4.6 ?mol/L, ale inhibice PMA-stimulované cPKC aktivity v intaktních buňkách vyžadovala nejméně pětkrát vyšší koncentracialkaloidu. Sanguinarin a chelerythrin bránily fosforylaci 80 kDa proteinu a zadržovaly 60 kDa fosfoproteid v cytosolu v koncentracích pod 10 ?mol/L. Navíc ani sanguinarin ani chelerythrin neblokovaly PMA-stimulovanou translokaci

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GP303%2F06%2FP193" target="_blank" >GP303/06/P193: Changes of expression of pro-inflammatory enzymes caused by isoquinoline alkaloids during differentiation of promyelocytes into neutrophils</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Biology and Toxicology

ISSN

0742-2091

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

15

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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