An evidence for regulatory cross-talk between aryl hydrocarbon receptor and glucocorticoid receptor in HepG2 cells.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F08%3A00006993" target="_blank" >RIV/61989592:15110/08:00006993 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/08:00109263

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

An evidence for regulatory cross-talk between aryl hydrocarbon receptor and glucocorticoid receptor in HepG2 cells.

Original language description

Aryl hydrocarbon receptor (AhR) and glucocorticoid receptor (GR) play crucial role in the regulation of drug metabolizing enzymes and in many essential physiological processes. Cellular signaling by these receptors shares several functional and regulatory features. Here we investigated regulatory cross-talk between these two receptors. Human hepatoma cells (HepG2) were the model of choice. We analyzed the effects of dexamethasone (DEX) and dioxin (TCDD) on i) expression of AhR and GRalpha mRNAs; ii) levels of AhR and GR proteins; iii) transcriptional activities of AhR and GR in reporter assays; iv) 7-ethoxyresorufin-O-deethylase activity (EROD). We found that both DEX and TCDD affected AhR and GR mRNAs expression, proteins levels and transcriptional activities in HepG2 cells. These effects on cellular signaling by AhR and GR comprised up-/down-regulation of gene expression and ligand-dependent protein degradation. We conclude that interactive regulatory cross-talk between GR and AhR re

Czech name

Důkaz pro regulační cross-talk mezi aryl uhlovodíkovým a glukokortikoidním receptorem v HepG2 buňkách

Czech description

Aryl uhlovodíkový receptor (AhR) a glukokortikoidní receptor (GR) mají klíčovou roli v regulaci enzymů metabolizujících léčiva. Analyzovali jsme vliv dexamethasonu a dioxinu na i) expresi mRNA v AhR a GR; ii) hladiny AhR a GR proteinů; iii) transkripčníaktivitu AhR a GR; iv) 7-ethoxyresorufin-O-deethylasovou aktivitu. Nalezli jsem, že jak dexamethason tak dioxin ovlivňovaly u AhR a GR expresi mRNA, hladinu AhR a GR proteinů, a transkripční aktivitu v HepG2 buňkách. Z výsledků vyplývá, že interaktivní regulační cross-talk mezi receptory AhR a GR má potenciální implikace ve fyziologii a metabolismu léčiv.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA303%2F07%2F0128" target="_blank" >GA303/07/0128: Study on tissue-specific aspects of transcriptional regulation of selected P-450 enzymes and drug transporters.</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research (print)

ISSN

0862-8408

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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