The broad-spectrum caspase inhibitor Boc-Asp-CMK induces cell death in human leukaemia cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F08%3A00007390" target="_blank" >RIV/61989592:15110/08:00007390 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The broad-spectrum caspase inhibitor Boc-Asp-CMK induces cell death in human leukaemia cells

Original language description

Synthetic caspase inhibitors and particularly broad- spectrum caspase inhibitors can prevent cells from death or at least slow down cell death process and abrogate some apoptotic hallmarks (Kitanaka and Kuchino 1999). However, not all synthetic caspase inhibitors diminish cell death. We have found that the broad spectrum caspase inhibitor Boc-Asp-CMK induced cell death at micromolar concentrations in human leukaemia cells. Interestingly, low concentrations of Boc-Asp-CMK induced cell death with apoptotic hallmarks. Increasing concentrations of Boc-Asp-CMK led to necrotic cell death. The switch between apoptosis and necrosis seemed to depend upon the degree of inhibition of executioner caspases, including caspase-3/7 with Boc-Asp-CMK. Interestingly, caspase-3 processing was not inhibited even for the highest concentration of Boc-Asp-CMK used. We assume that toxic properties of Boc-Asp-CMK can be attributed to the chloromethylketone residuum in its molecule, as its analogue Boc-Asp-FMK w

Czech name

Širokospektrý kaspázový inhibitor Boc-Asp-CMK indukuje buněčnou smrt u lidských leukemických buněk

Czech description

Syntetické inhibitory kaspáz a z části také širokospektré kaspázové inhibitory mohou zabránit buněčné smrti nebo přinejmenším zpomalit proces buněčné smrti a zamezit některým apoptotickým projevům.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicology in Vitro

ISSN

0887-2333

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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