Protectivity of Blue Honeysuckle Extract against Oxidative Human Endothelial Cells and Rat Hepatocytes Damage.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F09%3A00009524" target="_blank" >RIV/61989592:15110/09:00009524 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Protectivity of Blue Honeysuckle Extract against Oxidative Human Endothelial Cells and Rat Hepatocytes Damage.

Original language description

The effect of Lonicera caerulea L. (Blue Honeysuckle) phenolic fraction (18.5 % anthocyanins) on cell viability and against oxidative damage in low density lipoproteins (oxLDL), in rat microsomes and in primary cultures of rat hepatocytes and human umbilical vein endothelial cells (HUVEC), was tested. The phenolic fraction was non-toxic to rat hepatocytes and HUVEC at tested concentrations (1 ? 1000 ?g/mL) and time intervals up to 24 h inclusive. Phenolic fraction inhibited rat liver microsomes peroxidation, induced by tert-butyl hydroperoxide (tBH), with IC50 values 160 ? 20 ?g/mL. The fraction at 0.5, 1.0 and 2.0 ?g/mL delayed LDL oxidation, induced by Cu2+, by 130 ? 20%, 200 ? 30% and 400 ? 10%, respectively. The treatment of HUVEC with oxidativelymodified LDL induced an increase in LDH leakage and TBARS formation, and resulted in lower formazan formation from MTT uptake, most pronounced for 200 ?g/mL (24h oxidation) after 2 h incubation. The protective effect of the phenolic

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/FT-TA3%2F024" target="_blank" >FT-TA3/024: Plant chemoprotectants - New food supplements for health and prevention of chronic diseases.</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Agricultural and Food Chemistry

ISSN

0021-8561

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

15

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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