Comparison of chemiluminesce and flow-cytometry for reactive oxygen species estimation in sperm suspension

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F09%3A00009819" target="_blank" >RIV/61989592:15110/09:00009819 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of chemiluminesce and flow-cytometry for reactive oxygen species estimation in sperm suspension

Original language description

Introduction: Sperm oxidative stress has been shown to detrimentally affect its lipid and protein content and to induce sperm DNA damage compromising sperm fertilizing ability. Superoxide, hydrogen peroxide and peroxynitrite are the common form of reactive oxygen species (ROS). Chemiluminescence using luminol measures both intracellular and extracellular ROS levels making it difficult to determine the source of the free radicals. Recently, flow-cytometry has been utilized to measure selectively intracellular ROS production with the added advantage of requiring smaller number of sperm. This study compared chemiluminescence and flow-cytometry in measuring sperm ROS production in a group of men within a large fertility clinic. Materials & methods: The study was approved by the Ethical Board of the Palacky University in Olomouc (CR). In order to included a broad spectrum of men the study recruited 166 men presenting with their partners for fertility assessment and 31 volunteers and sperm d

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FK - Gynaecology and obstetrics

OECD FORD branch

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Project

<a href="/en/project/1A8621" target="_blank" >1A8621: Oxidative stress in pathophysiology of male infertility.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Human Reproduction

ISSN

0268-1161

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

Suppl 1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

2

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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