Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10212108" target="_blank" >RIV/61989592:15110/10:10212108 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen

Original language description

OBJECTIVE: To determine the relative contribution of different cell types in washed sperm to the overall intracellular production of H(2)O(2) and peroxynitrite. SETTING: University hospital. MAIN OUTCOME MEASURE(S): Simultaneous estimates of global ROS production assessed by chemiluminescence assay compared with flow cytometric measurements. RESULT(S): The estimates of ROS with use of chemiluminescence positively correlated with the estimates of H(2)O(2) (r = 0.53) and peroxynitrite (r = 0.62) as assessed with flow cytometry. H(2)O(2) and peroxynitrite were measurable also in samples in which chemiluminescence did not detect measurable values. Increased production of H(2)O(2) by one cell type was associated with a relative increase in its peroxynitriteproduction.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FK - Gynaecology and obstetrics

OECD FORD branch

—

Project

<a href="/en/project/1A8621" target="_blank" >1A8621: Oxidative stress in pathophysiology of male infertility.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Fertility and Sterility

ISSN

0015-0282

e-ISSN

—

Volume of the periodical

94

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—