DETERMINING THE CONTRIBUTION OF DIFFERENT CELL TYPES IN SEMEN TO THE GLOBAL VALUES OF THE INTRACELLULAR OXIDANTS H2O2 AND PEROXYNITRITE USING FLOWCYTOMETRY
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F09%3A00009818" target="_blank" >RIV/61989592:15110/09:00009818 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
DETERMINING THE CONTRIBUTION OF DIFFERENT CELL TYPES IN SEMEN TO THE GLOBAL VALUES OF THE INTRACELLULAR OXIDANTS H2O2 AND PEROXYNITRITE USING FLOWCYTOMETRY
Original language description
Objective: Sperm, leukocytes and round cells contribute to total oxidants production in semen. The Chemiluminescence assay provides a global value of reactive oxygen species in semen but provides no information on the contribution of different cell types. In this study we used flowcytometry to assess the global intracellular levels of the oxidants H 2O 2 and peroxynitrite in semen and the relative contribution of each cell type, simultaneously. Design: Prospective study. Materials and Methods: Semen samples from 19 fertility men and 120 men undergoing fertility assessment were collected. After liquefaction, semen was centrifuged at 300 g for 7 min. The peletted cells were resuspended in phosphate buffered saline to achieve sperm concentration of 1.25 10 6/mL. Aliquots were incubated with DCFH-DA and DHR12 dyes to detect intracellular H 2O 2 and peroxynitrite, respectively, using flowcytometry. Gated analysis determined the relative contribution of sperm, leukocytes and round cells. Res
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FK - Gynaecology and obstetrics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1A8621" target="_blank" >1A8621: Oxidative stress in pathophysiology of male infertility.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Fertility and Sterility
ISSN
0015-0282
e-ISSN
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Volume of the periodical
92
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
2
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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