Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10212701" target="_blank" >RIV/61989592:15110/10:10212701 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/10:00355107 RIV/61388971:_____/10:00355107

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism

Original language description

Reactive oxygen species (ROS) originating from mitochondria are perceived as a factor contributing to cell aging. Silybin and dehydrosilybin, two polyphenolic compounds, display a plethora of biological effects generally ascribed to their known antioxidant capacity. When investigating the cytoprotective effects of these two compounds in the primary cell cultures of neonatal rat cardiomyocytes, we noted the ability of dehydrosilybin to de-energize the cells by monitoring JC-1 fluorescence. Experiments evaluating oxygen consumption and membrane potential revealed that dehydrosilybin uncouples the respiration of isolated rat heart mitochondria albeit with a much lower potency than synthetic uncouplers. We infer that the apparent uncoupler-like activity ofdehydrosilybin is the basis of its ROS modulation effect in neonatal rat cardiomyocytes and leads us to propose a hypothesis on natural ischemia preconditioning by dietary polyphenols.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA303%2F08%2F0658" target="_blank" >GA303/08/0658: Modulation of uncoupling protein UcP2 and mitochondrial bioenergetics in cardiomyocytes by natural substances</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Bioenergetics and Biomembranes

ISSN

0145-479X

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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