Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10213496" target="_blank" >RIV/61989592:15110/10:10213496 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Induction of apoptosis by A3 adenosine receptor agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide in human leukemia cells: a possible involvement of intracellular mechanism.

Original language description

The sensitivity of cancer cells which exhibit multidrug resistance phenotype to A3 adenosine receptor (A3AR) agonist N6-(3-iodobenzyl)-adenosine-5'-N-methylcarboxamide (IB-MECA) was studied. Methods: To establish direct relationship between P-glycoprotein (P-gp, ABCB1, MDR1) and IB-MECA, a straightforward method for precise estimation of intracellular level of this A3AR agonist was developed. Results: We subjected three human leukemia cell lines HL-60, K562, and K562/HHT to the treatment with micromolarconcentration of IB-MECA. Despite the fact that all cell lines used expressed A3AR, there was a large difference in sensitivity to IB-MECA. While HL-60 and K562 cells were almost equally sensitive, the K562/HHT cells, which exhibit a multidrug resistance phenotype due to overexpression of P-gp, were significantly more resistant. We found that the intracellular level of IB-MECA in K562/HHT cells was approximately ten times lower than those in HL-60 or K562 cells.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

—

Project

<a href="/en/project/NR9482" target="_blank" >NR9482: P-glycoprotein and its possible contribution to development of resistance to Imatinib in Bcr-Abl expressing cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Physiologica

ISSN

1748-1708

e-ISSN

—

Volume of the periodical

199

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—