Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-gamma 1 and -gamma 2 mRNAs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F10%3A10215624" target="_blank" >RIV/61989592:15110/10:10215624 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Evidence for differential effects of glucose and cycloheximide on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-) machinery members: Superinduction of PPAR-gamma 1 and -gamma 2 mRNAs

Original language description

RT-PCR study was conducted to reveal the effects of normal and high glucose concentration without or with oleate (0.3 mmol/l) on mRNA levels of peroxisome proliferator-activated receptor- (PPAR-)alfa, -gama1, -gama2, and peroxisome proliferator activatedreceptor-gama coactivator- (PGC-)1alfa and -1beta in HepG2 cells under low-serum condition. Significant decrease in PPAR-gama1 and PGC-1alfa mRNA levels to about 50 % was observed during the first 4 h incubation period. During the next 4 h period, bothPPAR-gama1 and PGC-1alfa mRNAs were partly but significantly restored in high glucose batches. The presence of the transcriptional inhibitor actinomycin D revealed a signifiant protective effect of excess glucose on mature PPAR-gama1 and PGC-1alfa mRNAs.Furthermore, PPAR-gama1 and -gama2 mRNAs were differentially superinduced 1.2-2.5 fold in cells upon the administration of the translational inhibitor cycloheximide.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/OC%20153" target="_blank" >OC 153: Fatty acid- and glucose-induced changes in gene expression of peroxisome proliferator-activated receptor (PPAR) signalling cascade molecules</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Biochimica Polonica

ISSN

0001-527X

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

2

Country of publishing house

PL - POLAND

Number of pages

7

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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