Induction of heme oxygenase-1 by Macleaya cordata extract and its constituent sanguinarine in RAW264.7 cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F12%3A33138582" target="_blank" >RIV/61989592:15110/12:33138582 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Induction of heme oxygenase-1 by Macleaya cordata extract and its constituent sanguinarine in RAW264.7 cells
Original language description
We examined whether M. cordata extract and its alkaloid constituents, protopine, allocryptopine, sanguinarine and chelerythrine activate Nrf2 signalling pathway which regulates expression of cytoprotective enzymes such as heme oxygenase-1 (HO-1) and thioredoxin 1. In murine RAW264.7 macrophages, M. cordata extract increased mRNA and protein levels of HO-1. Of the alkaloids examined, sanguinarine appeared to be responsible for these effects. Sanguinarine induced nuclear accumulation of Nrf2, increased the expression of Hmox1 gene encoding HO-1 and elevated HO-1 protein levels. Sanguinarine-induced Hmox1 mRNA expression was suppressed by pharmacologic inhibitor of p38 MAPK. Sanguinarine also elevated protein levels of HO-1 and thioredoxin 1 in primary cultures of human hepatocytes. We conclude that sanguinarine may increase the capacity of the enzymatic antioxidant defence system via activation of the p38 MAPK/Nrf2 pathway.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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