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Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F12%3A33140827" target="_blank" >RIV/61989592:15110/12:33140827 - isvavai.cz</a>

  • Alternative codes found

    RIV/68378050:_____/12:00387884

  • Result on the web

    <a href="http://dx.doi.org/10.4161/cc.19978" target="_blank" >http://dx.doi.org/10.4161/cc.19978</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.4161/cc.19978" target="_blank" >10.4161/cc.19978</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Ubiquitin-activating enzyme UBA1 is required for cellular response to DNA damage

  • Original language description

    The cellular DNA damage response (DDR) machinery that maintains genomic integrity and prevents severe pathologies, including cancer, is orchestrated by signaling through protein modifications. Protein ubiquitylation regulates repair of DNA double-strandbreaks (DSBs), toxic lesions caused by various metabolic as well as environmental insults such as ionizing radiation (IR). Whereas several components of the DSB-evoked ubiquitylation cascade have been identified, including RNF168 and BRCA1 ubiquitin ligases, whose genetic defects predispose to a syndrome mimicking ataxiatelangiectasia and cancer, respectively, the identity of the apical E1 enzyme involved in DDR has not been established. Here, we identify ubiquitin-activating enzyme UBA1 as the E1 enzyme required for responses to IR and replication stress in human cells. We show that siRNA-mediated knockdown of UBA1, but not of another UBA family member UBA6, impaired formation of both ubiquitin conjugates at the sites of DNA damage and

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    CELL CYCLE

  • ISSN

    1538-4101

  • e-ISSN

  • Volume of the periodical

    11

  • Issue of the periodical within the volume

    8

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    1573-1582

  • UT code for WoS article

    000303162100019

  • EID of the result in the Scopus database