The energetic difference between synthesis of correct and incorrect base pairs accounts for highly accurate DNA replication

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33143455" target="_blank" >RIV/61989592:15110/13:33143455 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/ja309866m" target="_blank" >http://dx.doi.org/10.1021/ja309866m</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ja309866m" target="_blank" >10.1021/ja309866m</a>

Result language
angličtina
Original language name
The energetic difference between synthesis of correct and incorrect base pairs accounts for highly accurate DNA replication
Original language description
To better understand the energetics of accurate DNA replication, we directly measured deltaG(o) for the incorporation of a nucleotide into elongating dsDNA in solution (deltaG(o)(incorporation)). Direct measurements of the energetic difference between synthesis of correct and incorrect base pairs found it to be much larger than previously believed (average deltadeltaG(o)(incorporation) = 5.2 +- 1.34 kcal mol(-1)). Importantly, these direct measurements indicate that deltadeltaG(o)(incorporation) alone can account for the energy required for highly accurate DNA replication. Evolutionarily, these results indicate that the earliest polymerases did not have to evolve sophisticated mechanisms to replicate nucleic acids; they may only have had to take advantage of the inherently more favorable deltaG(o) for polymerization of correct nucleotides. These results also provide a basis for understanding how polymerases replicate DNA (or RNA) with high fidelity.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Project
<a href="/en/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicine for regional development and human resources</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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