Acetylation dynamics of human nuclear proteins during the ionizing radiation-induced DNA damage response

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33145212" target="_blank" >RIV/61989592:15110/13:33145212 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.4161/cc.24758" target="_blank" >http://dx.doi.org/10.4161/cc.24758</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4161/cc.24758" target="_blank" >10.4161/cc.24758</a>

Result language

angličtina

Original language name

Acetylation dynamics of human nuclear proteins during the ionizing radiation-induced DNA damage response

Original language description

Genotoxic insults, such as ionizing radiation (IR), cause DNA damage that evokes a multifaceted cellular DNA damage response (DDR). DNA damage signaling events that control protein activity, subcellular localization, DNA binding, protein-protein interactions, etc. rely heavily on time-dependent posttranslational modifications (PTMs). To complement our previous analysis of IR-induced temporal dynamics of nuclear phosphoproteome, we now identify a range of human nuclear proteins that are dynamically regulated by acetylation, and predominantly deacetylation, during IR-induced DDR by using mass spectrometry-based proteomic approaches. Apart from cataloging acetylation sites through SILAC proteomic analyses before IR and at 5 and 60 min after IR exposure ofU2OS cells, we report that: (1) key components of the transcriptional machinery, such as EP300 and CREBBP, are dynamically acetylated; (2) that nuclear acetyltransferases themselves are regulated, not on the protein abundance level, but

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

<a href="/en/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicine for regional development and human resources</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Cycle

ISSN

1551-4005

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

11

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

1688-1695

UT code for WoS article

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EID of the result in the Scopus database

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