Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33153310" target="_blank" >RIV/61989592:15110/13:33153310 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s11046-013-9695-3" target="_blank" >http://dx.doi.org/10.1007/s11046-013-9695-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11046-013-9695-3" target="_blank" >10.1007/s11046-013-9695-3</a>

Result language
angličtina
Original language name
Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR
Original language description
Pythiosis is a rare infectious disease caused by Pythium insidiosum, which typically occurs in tropical and subtropical regions. The high mortality rate may be in consequence of the lack of diagnosis. The objective of this study was to evaluate reliability of a new single-tube nested PCR for detection of P. insidiosum DNA. A total of 78 clinical isolates of various fungi and bacteria, 106 clinical specimens and 80 simulated positive blood samples were tested. The developed primer pairs CPL6-CPR8 and YTL1-YTR1 are located on 18S subunit of the rRNA gene of P. insidiosum. The specificity, negative and positive predictive values were 100, 100 and 87.5 %, respectively, as compared with direct microscopy and cultivation. The detection limit of the single-tube nested PCR was 21 zoospores corresponding to 2.7 pg of the DNA. The results demonstrate that the new single-tube nested PCR offers a highly sensitive, specific and rapid genetic method for detecting P. insidiosum.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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