Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F14%3A33148768" target="_blank" >RIV/61989592:15110/14:33148768 - isvavai.cz</a>

Alternative codes found

RIV/61389005:_____/14:00427322

Result on the web

<a href="http://dx.doi.org/10.1016/j.fsigen.2013.10.009" target="_blank" >http://dx.doi.org/10.1016/j.fsigen.2013.10.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fsigen.2013.10.009" target="_blank" >10.1016/j.fsigen.2013.10.009</a>

Result language

angličtina

Original language name

Fluorescent Random Amplified Microsatellites (F-RAMS) analysis of mushrooms as a forensic investigative tool

Original language description

The capability of Fluorescent Random Amplified Microsatellites (F-RAMS) to profile hallucinogenic mushrooms to species and sub-species level was assessed. Fifteen samples of Amanita rubescens and 22 samples of other hallucinogenic and non-hallucinogenicmushrooms of the genera Amanita and Psilocybe were profiled using two fluorescently-labeled, 50degenerate primers, 50-6FAM-SpC3-DD (CCA)5 and 50- 6FAM-SpC3-DHB (CGA)5, which target different microsatellite repeat regions. Among the two primers, 50-6FAM-SpC3-DHB (CGA)5 provided more reliable data for identification purposes, by grouping samples of the same species and clustering closely related species together in a dendrogram based on amplicon similarities. A high degree of intra-specific variation between the 15 A. rubescens samples was shown with both primers and the amplicons generated for all A. rubescens samples were organized into three classes of amplicons (discriminant, private, and marker) based on their individualizing potenti

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicine for regional development and human resources</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Forensic Science International-Genetics

ISSN

1872-4973

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

duben 2014

Country of publishing house

IE - IRELAND

Number of pages

8

Pages from-to

25-32

UT code for WoS article

000331834600019

EID of the result in the Scopus database

—