DMT1-Mutant Erythrocytes have Shortened Life Span, Accelerated Glycolysis and Increased Oxidative Stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F14%3A33150961" target="_blank" >RIV/61989592:15110/14:33150961 - isvavai.cz</a>

Result on the web

<a href="http://www.karger.com/Article/FullText/369665" target="_blank" >http://www.karger.com/Article/FullText/369665</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000369665" target="_blank" >10.1159/000369665</a>

Result language

angličtina

Original language name

DMT1-Mutant Erythrocytes have Shortened Life Span, Accelerated Glycolysis and Increased Oxidative Stress

Original language description

Deficiency of the divalent metal transporter 1 (DMT1) leads to hypochromic microcytic anemia. We have previously shown that DMT1 deficiency impairs erythroid differentiation and induces apoptosis of erythroid cells. Here we analyzed metabolic processes and survival of mature erythrocytes in order to address potential involvement of erythrocyte defect in the pathophysiology of the disease. We observed an accelerated clearance of CFSE-labeled DMT1-mutant erythrocytes from circulating blood when compared to wild-type erythrocytes. In vitro, DMT1-mutant erythrocytes showed significantly increased Annexin V binding after exposure to hyperosmotic shock and glucose depletion. Despite exaggerated anti-oxidative defense, higher ROS levels were present in DMT1-mutant erythrocytes. Accelerated anaerobic glycolysis and reduced ATP/ADP ratio detected in DMT1- mutant erythrocytes indicate enhanced demand for ATP. We propose that DMT1 deficiency negatively affects metabolism and life span of mature e

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cellular Physiology and Biochemistry

ISSN

1015-8987

e-ISSN

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Volume of the periodical

34

Issue of the periodical within the volume

6

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

2221-2231

UT code for WoS article

000347449100033

EID of the result in the Scopus database

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