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TRACKING MITOCHODNRIAL DNA IN SITU

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33154081" target="_blank" >RIV/61989592:15110/15:33154081 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1007/978-1-4939-3040-1" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-3040-1</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-4939-3040-1" target="_blank" >10.1007/978-1-4939-3040-1</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    TRACKING MITOCHODNRIAL DNA IN SITU

  • Original language description

    The methods of the detection of (1) non-labeled and (2) BrdU-labeled mitochondrial DNA (mtDNA) are described. They are based on the production of singlet oxygen by monovalent copper ions and the subsequent induction of DNA gaps. The ends of interrupted DNA serve as origins for the labeling of mtDNA by DNA polymerase I or they are utilized by exonuclease that degrades DNA strands, unmasking BrdU in BrdU-labeled DNA. Both methods are sensitive approaches without the need of additional enhancement of the signal or the use of highly sensitive optical systems.

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    Mitochondrial DNA: Methods and Protocols

  • ISBN

    978-1-4939-3039-5

  • Number of pages of the result

    12

  • Pages from-to

    81-92

  • Number of pages of the book

    234

  • Publisher name

    Humana Press, Springer Science+Business Media

  • Place of publication

    New York

  • UT code for WoS chapter