TRACKING MITOCHODNRIAL DNA IN SITU
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33154081" target="_blank" >RIV/61989592:15110/15:33154081 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-3040-1" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-3040-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-3040-1" target="_blank" >10.1007/978-1-4939-3040-1</a>
Alternative languages
Result language
angličtina
Original language name
TRACKING MITOCHODNRIAL DNA IN SITU
Original language description
The methods of the detection of (1) non-labeled and (2) BrdU-labeled mitochondrial DNA (mtDNA) are described. They are based on the production of singlet oxygen by monovalent copper ions and the subsequent induction of DNA gaps. The ends of interrupted DNA serve as origins for the labeling of mtDNA by DNA polymerase I or they are utilized by exonuclease that degrades DNA strands, unmasking BrdU in BrdU-labeled DNA. Both methods are sensitive approaches without the need of additional enhancement of the signal or the use of highly sensitive optical systems.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Mitochondrial DNA: Methods and Protocols
ISBN
978-1-4939-3039-5
Number of pages of the result
12
Pages from-to
81-92
Number of pages of the book
234
Publisher name
Humana Press, Springer Science+Business Media
Place of publication
New York
UT code for WoS chapter
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