Polyethylenimine architecture-dependent metabolic imprints and perturbation of cellular redox homeostasis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33154162" target="_blank" >RIV/61989592:15110/15:33154162 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bbabio.2014.12.002" target="_blank" >http://dx.doi.org/10.1016/j.bbabio.2014.12.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbabio.2014.12.002" target="_blank" >10.1016/j.bbabio.2014.12.002</a>

Result language
angličtina
Original language name
Polyethylenimine architecture-dependent metabolic imprints and perturbation of cellular redox homeostasis
Original language description
Polyethylenimines (PEIs) are among the most efficient polycationic non-viral transfectants. PEI architecture and size not only modulate transfection efficiency, but also cytotoxicity. However, the underlying mechanisms of PEI-induced multifaceted cell damage and death are largely unknown. Here, we demonstrate that the central mechanisms of PEI architecture- and size-dependent perturbations of integrated cellular metabolomics involve destabilization of plasma membrane and mitochondrial membranes with consequences on mitochondrial oxidative phosphorylation (OXPHOS), glycolytic flux and redox homeostasis that ultimately modulate cell death. In comparison to linear PEI, the branched architectures induced greater plasma membrane destabilization and were more detrimental to glycolytic activity and OXPHOS capacity as well as being a more potent inhibitor of the cytochrome c oxidase. Accordingly, the branched architectures caused a greater lactate dehydrogenase (LDH) and ATP depletion, activat
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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