Differential Regulation of Proinflammatory Mediators Following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33154232" target="_blank" >RIV/61989592:15110/15:33154232 - isvavai.cz</a>
Result on the web
<a href="http://www.hindawi.com/journals/bmri/2015/292851/" target="_blank" >http://www.hindawi.com/journals/bmri/2015/292851/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2015/292851" target="_blank" >10.1155/2015/292851</a>

Result language
angličtina
Original language name
Differential Regulation of Proinflammatory Mediators Following LPS- and ATP-Induced Activation of Monocytes from Patients with Antiphospholipid Syndrome
Original language description
Antiphospholipid syndrome (APS) is an acquired autoimmune disorder characterized by recurrent thrombosis and pregnancy morbidity in association with the presence of antiphospholipid antibodies. Growing evidence supports the involvement of monocytes in APS pathogenesis. Inflammatory activation of monocytes promotes thrombus formation and other APS complications. However, mechanisms underlying their activation are poorly investigated. We aimed to determine transcriptional activity of monocytes after exposing them to low concentrations of lipopolysaccharide (LPS) and LPS + adenosine triphosphate (ATP) using comparative qRT-PCR. The results showed that LPS significantly increased transcriptional levels of TLR2, IL-23, CCL2, CXCL10, IL-1?, and IL-6 in APS cells, while, in cells from healthy donors, LPS resulted in IL-6 and STAT3 elevated mRNAs. Double stimulation of the cells resulted in decreased mRNA levels of NLRP3 in monocytes isolated from healthy donors and CCL2, IL-1? in APS cells. B
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EC - Immunology
OECD FORD branch
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Project
<a href="/en/project/EE2.3.30.0004" target="_blank" >EE2.3.30.0004: POST-UP</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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