Immunomodulatory Effects of Therapeutic Plasma Exchange on Monocytes in Antiphospholipid Syndrome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33156191" target="_blank" >RIV/61989592:15110/16:33156191 - isvavai.cz</a>
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950391/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4950391/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/etm.2016.3441" target="_blank" >10.3892/etm.2016.3441</a>
Alternative languages
Result language
angličtina
Original language name
Immunomodulatory Effects of Therapeutic Plasma Exchange on Monocytes in Antiphospholipid Syndrome
Original language description
The antiphospholipid syndrome (APS) is a systemic autoimmune disorder characterized by thrombosis and recurrent fetal loss, with the persistent presence of antiphospholipid antibodies (aPL). aPL exert their pathogenic effect via overproduction of tissue factor and activation of complement and several cell types, such as endothelia, platelets and importantly also monocytes. As a result, a hypercoagulable state develops leading to APS-associated obstetric complications and fetal loss. Despite far from optimal, treatment of APS usually includes heparin and low dose aspirin. Recently, successful application of plasma exchange (PE) therapy in APS patients with obstetric complications who failed the above standard treatment has been described. In this study we, therefore, investigated mechanism of PE action, namely we explored whether PE affects the functional activity of APS monocytes as assessed by expression of 11 mRNA transcripts (cytokines, signaling molecules/transcription factors). Monocytes were collected before and after the PE course from women with APS who experienced recurrent pregnancy losses as well as healthy volunteers. Compared with control cells, APS monocytes showed deregulated expression of IL-1β, IL-6, IL-23, CCL2, CXCL10 TLR2, and STAT3 at baseline. PE resulted in increased IL-1β, IL-6, IL-23, CCL2, P2X7 and TNFα mRNA transcripts in APS monocytes which returned to normal ranges, similar to those observed in healthy control cells. Furthermore, PE therapy counterbalanced the expression levels of CCL2 and CXCL10 which levels are indicative of Th1/Th2 balance. Thus, our findings provide evidence that primarily altered transcriptional profile of APS monocytes is restored due to immunomodulatory effect of plasmapheresis.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE2.3.30.0041" target="_blank" >EE2.3.30.0041: POST-UP II.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Experimental and Therapeutic Medicine
ISSN
1792-0981
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
2
Country of publishing house
GR - GREECE
Number of pages
7
Pages from-to
1189-1195
UT code for WoS article
000380278900104
EID of the result in the Scopus database
2-s2.0-84974739742