Fast and sensitive HPLC method for the determination of neopterin, kynurenine and tryptophan in amniotic fluid, malignant effusions and wound exudates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33155485" target="_blank" >RIV/61989592:15110/15:33155485 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/15:10312560 RIV/00179906:_____/15:10312560

Result on the web

<a href="http://dx.doi.org/10.4155/bio.15.175" target="_blank" >http://dx.doi.org/10.4155/bio.15.175</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4155/bio.15.175" target="_blank" >10.4155/bio.15.175</a>

Result language

angličtina

Original language name

Fast and sensitive HPLC method for the determination of neopterin, kynurenine and tryptophan in amniotic fluid, malignant effusions and wound exudates

Original language description

Aim: A new HPLC method for the determination of neopterin, kynurenine and tryptophan using a second-generation monolith stationary phase and high-throughput sample preparation procedure based on microplates was developed and fully validated. Materials &methods: As the stationary phase a monolithic C18 Chromolith high-resolution column with dimensions of 4.6 x 100 mm connected to a monolithic 4.6 x 10-mm security guard was used. Separation was achieved using 15 mM phosphate buffer (KH2PO4 + K2HPO4 center dot 3H(2)O at pH 3) and acetonitrile in gradient mode. Results: Target analytes were determined in 5.5 minutes in amniotic fluid, effusions and wound exudates with a limit of quantification (LOQ) of 1.25 nM for neopterin, 2.5 mu M for tryptophan and 0.25 mu M for kynurenine. Discussion: The method was applied to real clinical sample measurements, and it will be used to monitor neopterin, kynurenine and tryptophan levels in biological fluids to assess the patient response to therapy and

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioanalysis

ISSN

1757-6180

e-ISSN

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Volume of the periodical

7

Issue of the periodical within the volume

21

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

2751-2762

UT code for WoS article

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EID of the result in the Scopus database

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