Simultaneous determination of mushroom toxins ?-amanitin, ?-amanitin and muscarine in human urine by solid-phase extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33155700" target="_blank" >RIV/61989592:15110/15:33155700 - isvavai.cz</a>
Alternative codes found
RIV/00098892:_____/15:#0000975
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S037907381500153" target="_blank" >http://www.sciencedirect.com/science/article/pii/S037907381500153</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.forsciint.2015.04.007" target="_blank" >10.1016/j.forsciint.2015.04.007</a>

Result language
angličtina
Original language name
Simultaneous determination of mushroom toxins ?-amanitin, ?-amanitin and muscarine in human urine by solid-phase extraction and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry
Original language description
This paper presents a method for the simultaneous determination of ?-amanitin, ?-amanitin and muscarine in human urine by solid-phase extraction (SPE) and ultra-high-performance liquid chromatography coupled with ultra-high-resolution TOF mass spectrometry. The method can be used for a diagnostics of mushroom poisonings. Different SPE cartridges were tested for sample preparation, namely hydrophilic modified reversed-phase (Oasis HLB) and polymeric weak cation phase (Strata XCW).The latter gave better results and therefore it was chosen for the subsequent method optimization and partial validation. In the course of validation, limits of detection, linearity, intraday and interday precisions and recoveries were evaluated. The obtained LOD values of ?-amanitin and ?-amanitin were1 ng/mL and of muscarine 0.09 ng/mL. The intraday and interday precisions of human urine spiked with ?-amanitin (10 ng/mL), ?-amanitin (10 ng/mL) and muscarine (1 ng/mL) ranged from 6% to 10% and from 7% to 13%,
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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