Determination of muscarine in human urine by electrospray liquid chromatographic-mass spectrometric

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F11%3A00102032" target="_blank" >RIV/00843989:_____/11:00102032 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/11:33118206 RIV/61989592:15310/11:33118206
Result on the web
<a href="http://dx.doi.org/10.1016/j.jchromb.2011.07.009" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2011.07.009</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jchromb.2011.07.009" target="_blank" >10.1016/j.jchromb.2011.07.009</a>

Result language
angličtina
Original language name
Determination of muscarine in human urine by electrospray liquid chromatographic-mass spectrometric
Original language description
A liquid chromatography-mass spectrometry based method for determination of muscarine in human urine was developed and validated. The method involved a solid phase extraction of muscarine from urine using Strata X-CW column. Separation of muscarine was achieved within 16.0 min on a reversed phase Gemini C18 analytical column (150 mm x 2.0 mm id., 5 mu m) with a mobile phase consisted of aqueous 8 mmol/L heptafluorobutyric acid and acetonitrile in a gradient mode. Mass spectrometric detection was performed at m/z 174 and m/z 216 for muscarine and acetylmuscarine (internal standard), respectively. The linearity was satisfactory with a coefficient of determination (R(2)) 0.9993 at concentration range from 0.3 ng/mL to 2.0 mu g/mL. LOD and LOQ for muscarine was 0.09 ng/mL and 0.3 ng/mL, respectively. The found out recoveries of muscarine were 96% or 95% for concentration 0.3 ng/mL and 0.2 mu g/mL or 2.0 mu g/mL, respectively. The precision in the intra-assay-study varied from 0.48% to 1.39
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
<a href="/en/project/NS10269" target="_blank" >NS10269: The Objective Analytical Method for Amanita Intoxication Diagnosis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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