Effect of silver nanoparticles on primary cell cultures of fibroblasts and keratinocytes in a wound-healing model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33159275" target="_blank" >RIV/61989592:15110/16:33159275 - isvavai.cz</a>

Result on the web

<a href="http://www.jab-fm.com/article/d2c3d2d2-be8b-49d1-9e59-bcb1f49f0f5f" target="_blank" >http://www.jab-fm.com/article/d2c3d2d2-be8b-49d1-9e59-bcb1f49f0f5f</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5301/jabfm.5000268" target="_blank" >10.5301/jabfm.5000268</a>

Result language

angličtina

Original language name

Effect of silver nanoparticles on primary cell cultures of fibroblasts and keratinocytes in a wound-healing model

Original language description

Background: Nanoparticles are widely used in different technological fields, one of which is medicine. Because of their antibacterial properties, silver nanoparticles (AgNPs) are used in several types of wound dressings for the treatment of burns and nonhealing wounds, but their influence on each component of the wound-healing process remains unclear. In the present study, we evaluated the effects of AgNPs on normal human dermal fibroblasts (NHDFs) and normal human epidermal keratinocytes (NHEKs). Both cell types are important for wound healing, including with regard to inflammation, proliferation and tissue remodeling. Each phase of wound healing can be characterized by the secretion of cytokines, chemokines and growth factors. Methods: The production of inflammatory parameters (tumor necrosis factor α [TNF-α], interleukin-6 [IL-6], IL-8 and IL-12 and cyclooxygenase-2 [COX-2]), angiogenesis parameters (vascular endothelial growth factor [VEGF], granulocyte macrophage colony-stimulating factor) and matrix metalloproteinases (MMP-1, MMP-2, MMP-3 and MMP-9) by NHDFs and NHEKs were examined by ELISA or Western blot after 24 and 48 hours of incubation with AgNPs. Results: We found that AgNPs decreased some inflammatory cytokines (TNF-α and IL-12) and growth factors (VEGF) that were produced by NHDFs and NHEKs after 24 and 48 hours and decreased the expression of COX-2 after 24 hours but only at the highest concentration of AgNPs (25 parts per million). Conclusions: The results indicate that NHEKs are more susceptible to the application of AgNPs than NHDFs, and AgNPs may be useful for medical applications for the treatment of wounds.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Biomaterials &amp; Functional Materials

ISSN

2280-8000

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

2

Country of publishing house

IT - ITALY

Number of pages

6

Pages from-to

"e-137"-"e-142"

UT code for WoS article

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EID of the result in the Scopus database

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