Possibility of Coagulation System Activation Determination with Tissue Factor in Pregnancy Complications
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33159832" target="_blank" >RIV/61989592:15110/16:33159832 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15120/16:33159832
Result on the web
<a href="http://dx.doi.org/10.7754/Clin.Lab.2016.151234" target="_blank" >http://dx.doi.org/10.7754/Clin.Lab.2016.151234</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.7754/Clin.Lab.2016.151234" target="_blank" >10.7754/Clin.Lab.2016.151234</a>
Alternative languages
Result language
angličtina
Original language name
Possibility of Coagulation System Activation Determination with Tissue Factor in Pregnancy Complications
Original language description
Background: In this part of the study, where we determined the causes of preeclampsia and other obstetric complications, we focused on the role of tissue factor (TF) in the activation of these pathophysiological processes. Recent findings attribute a significant part of the activation of coagulation creation of autoantibodies. Once this mechanism is activated, the antibodies induce expression of tissue factor (TF, CD142) on monocytes and vascular endothelial cells. Methods: We have proposed a monitor activation model of the coagulation system in preeclampsia and other pregnancy complications using TF expression on monocytes by flow cytometry and simultaneous determination the TF-induced thrombin generation in plasma. To determine expression of tissue factor (CD142) on monocytes, we proposed a method of multicolor flow cytometry using anti CD45 PerCP, anti CD14 APC, anti CD16b FITC, and anti CD142 PE antibodies and the corresponding isotype controls. Results: We verified the model on patients with severe antiphospholipid syndrome, which is a high expression of antibodies, in particular against beta-2GPI. Conclusions: We demonstrated complete inhibition of TF expression on monocytes and a significant reduction of thrombin generation in plasma.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT14394" target="_blank" >NT14394: Tissue factor activation in pregnancy complications</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinical Laboratory
ISSN
1433-6510
e-ISSN
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Volume of the periodical
62
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
1851-1856
UT code for WoS article
000385336100003
EID of the result in the Scopus database
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