Metabolic Response of Visceral White Adipose Tissue of Obese Mice Exposed for 5 Days to Human Room Temperature Compared to Mouse Thermoneutrality

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F17%3A73580147" target="_blank" >RIV/61989592:15110/17:73580147 - isvavai.cz</a>

Result on the web

<a href="https://obd.upol.cz/id_publ/333160007" target="_blank" >https://obd.upol.cz/id_publ/333160007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fphys.2017.00179" target="_blank" >10.3389/fphys.2017.00179</a>

Result language

angličtina

Original language name

Metabolic Response of Visceral White Adipose Tissue of Obese Mice Exposed for 5 Days to Human Room Temperature Compared to Mouse Thermoneutrality

Original language description

Housing of laboratory mice at room temperature (RT) might be considered a constant cold stress, which induces a thermogenic program in brown adipose tissue. However, the early adaptive response of white adipose tissue, the fat storage organ of the body, to a change from thermoneutrality to RT is not known. This was investigated here for various WAT depots, focusing on epididymal WAT. Male adult diet-induced obese C57BL/6JOlaHsd mice housed at thermoneutrality, were for five days either switched to RT or remained at thermoneutrality. Energy metabolism was continuously measured using indirect calorimetry. At the end of the study, serum metabolomics and WAT transcriptomics were performed. We confirmed activation of the thermogenic program in 22ºC housed mice. Body weight and total fat mass were reduced. Whole body energy expenditure was increased, with a higher fatty acid to carbohydrate oxidation ratio and increased serum acylcarnitine levels, while energy intake was not significantly different between the two groups. Transcriptome analysis of eWAT identified tissue remodelling and inflammation as the most affected processes. Expression of pro-inflammatory M1 macrophage-related genes, and M1 over M2 macrophage ratio were decreased, which might be linked to an increased insulin sensitivity. Markers of thermogenesis were not altered in eWAT. Decreased expression of tryptophan hydroxylase 2 and cholecystokinin might represent altered neuroendocrine signalling. eWAT itself does not show increased fatty acid oxidation. The three measured WATs, epididymal, mesenteric, and retroperitoneal, showed mainly similar responses; reduced inflammation, decreased carbohydrate oxidation, and no or small differences in fatty acid oxidation. However, Ucp1 was only expressed and increased in rWAT in 22ᵒC housed mice. Cck expression was decreased in the three WATs, significantly in eWAT and rWAT, in contrast to Tph2, which was decreased in eWAT while not expressed in mWAT and rWAT.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

<a href="/en/project/LO1304" target="_blank" >LO1304: Support of suistainability of the Institute of Molecular and Translational Medicine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Physiology

ISSN

1664-042X

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

179 (23 March 2017)

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

1-15

UT code for WoS article

000397087800002

EID of the result in the Scopus database

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