White adipose tissue response of obese mice to ambient oxygen restriction at thermoneutrality: response markers identified, but no WAT inflammation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73594177" target="_blank" >RIV/61989592:15110/19:73594177 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2073-4425/10/5/359/htm" target="_blank" >https://www.mdpi.com/2073-4425/10/5/359/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/genes10050359" target="_blank" >10.3390/genes10050359</a>

Result language

angličtina

Original language name

White adipose tissue response of obese mice to ambient oxygen restriction at thermoneutrality: response markers identified, but no WAT inflammation

Original language description

Obesity is associated with white adipose tissue (WAT) hypoxia and inflammation. We aimed to test whether mild environmental oxygen restriction (OxR, 13% O2), imposing tissue hypoxia, triggers WAT inflammation in obese mice. Thirteen weeks diet-induced obese male adult C57BL/6JOlaHsd mice housed at thermoneutrality were exposed for five days to OxR versus normoxia. WAT and blood were isolated and used for analysis of metabolites and adipokines, WAT histology and macrophage staining, and WAT transcriptomics. OxR increased circulating levels of haemoglobin and haematocrit as well as hypoxia responsive transcripts in WAT and decreased blood glucose, indicating systemic and tissue hypoxia. WAT aconitase activity was inhibited. Macrophage infiltration as marker for WAT inflammation tended to be decreased, which was supported by down regulation of inflammatory genes S100a8, Ccl8, Clec9a, Saa3, Mgst2, and Saa1. Other down regulated processes include cytoskeleton remodelling and metabolism, while response to hypoxia appeared most prominently up regulated. The adipokines coiled-coil domain containing 3 (CCDC3) and adiponectin, as well as the putative WAT hormone cholecystokinin (CCK), were reduced by OxR on transcript (Cck, Ccdc3) and/or serum protein level (adiponectin, CCDC3). Conclusively, our data demonstrate that also in obese mice OxR does not trigger WAT inflammation. However, OxR does evoke a metabolic response in WAT, with CCDC3 and adiponectin as potential markers for systemic or WAT hypoxia.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genes

ISSN

2073-4425

e-ISSN

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Volume of the periodical

359

Issue of the periodical within the volume

10

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

"nestránkováno"

UT code for WoS article

000470964100038

EID of the result in the Scopus database

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