The effect of cytokinins on growth, phenolics, antioxidant and antimicrobial potential in liquid agitated shoot cultures of Knautia sarajevensis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F17%3A73584133" target="_blank" >RIV/61989592:15110/17:73584133 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/17:73584133 RIV/00027006:_____/17:00004112
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs11240-017-1288-2" target="_blank" >https://link.springer.com/article/10.1007%2Fs11240-017-1288-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11240-017-1288-2" target="_blank" >10.1007/s11240-017-1288-2</a>
Alternative languages
Result language
angličtina
Original language name
The effect of cytokinins on growth, phenolics, antioxidant and antimicrobial potential in liquid agitated shoot cultures of Knautia sarajevensis
Original language description
Endemic plants are under constant threat of extinction and micropropagation protocols that can provide not only mechanisms for their revitalisation, but also a possibility for potential sources of new biologically active substances. This study describes the very first use of the liquid culture system for in vitro Knautia sarajevensis shoot multiplication, production of biomass, and an increase in biological activities of the extracts. Murashige and Skoog media containing various concentrations of cytokinins (6-benzyladenine, zeatin, and kinetin) were used to establish agitated shoot cultures of this endemic plant species and to evaluate their effect on shoot morphology. HPLC analysis of phenolic compounds show that the main metabolite in all extracts was salicylic acid with accumulation of rosmarinic and 4-hydroxybenzoic acid for some treatments. The richest sources of phenolics were shoots cultivated in media containing zeatin, which also had high influence on biomass production. Analysis of antioxidant and antimicrobial potential suggests that this plant could have beneficial biological activities. Methanol extracts of shoots cultivated in media containing 2.0 mg/L 6-benzyladenine were moderately active against Staphylococcus aureus and Bacillus spizizeni. DPPH assay showed radical scavenging activity of shoot cultures with IC50 10–90 μg/mL.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell, Tissue and Organ Culture (print)
ISSN
0167-6857
e-ISSN
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Volume of the periodical
131
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
347-357
UT code for WoS article
000412689000013
EID of the result in the Scopus database
2-s2.0-85026748423