Imaging of Pseudomonas aeruginosa infection with Ga-68 labelled pyoverdine for positron emission tomography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73590728" target="_blank" >RIV/61989592:15110/18:73590728 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/18:00496187 RIV/61989592:15310/18:73590728
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pubmed/?term=Imaging+of+Pseudomonas+aeruginosa+infection+with+Ga-68+labelled+pyoverdine+for+positron+emission+tomography" target="_blank" >https://www.ncbi.nlm.nih.gov/pubmed/?term=Imaging+of+Pseudomonas+aeruginosa+infection+with+Ga-68+labelled+pyoverdine+for+positron+emission+tomography</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-018-33895-w" target="_blank" >10.1038/s41598-018-33895-w</a>
Alternative languages
Result language
angličtina
Original language name
Imaging of Pseudomonas aeruginosa infection with Ga-68 labelled pyoverdine for positron emission tomography
Original language description
Pseudomonas aeruginosa is an increasingly prevalent opportunistic pathogen that causes a variety of life-threatening nosocomial infections. Novel strategies for the development of new antibacterial treatments as well as diagnostic tools are needed. One of the novel diagnostic strategies for the detection of infection could be the utilization of siderophores. Siderophores are low-molecular-weight chelators produced by microbes to scavenge essential iron. Replacing iron in siderophores by suitable radiometals, such as Ga-68 for positron emission tomography (PET) imaging, opens approaches for targeted imaging of infection. Here we report on pyoverdine PAO1 (PVD-PAO1), a siderophore produced by P. aeruginosa, labelled with Ga-68 for specific imaging of Pseudomonas infections. PVD-PAO1 was labelled with Ga-68 with high radiochemical purity. The resulting complex showed hydrophilic properties, low protein binding and high stability in human serum. In vitro uptake of Ga-68-PVD-PAO1 was highly dependent on the type of microbial culture. In normal mice Ga-68-PVD-PAO1 showed rapid pharmacokinetics with urinary excretion. PET imaging in infected animals displayed specific accumulation of Ga-68-PVD-PAO1 in infected tissues and better distribution than clinically used F-18-fluorodeoxyglucose (F-18-FDG) and Ga-68-citrate. Ga-68 labelled pyoverdine PAO1 seems to be a promising agent for imaging of P. aeruginosa infections by means of PET.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10700 - Other natural sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
OCT 24 2018
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
"nestránkováno"
UT code for WoS article
000448109000023
EID of the result in the Scopus database
2-s2.0-85055459467