Effect of the amino acid substitution in the DNA-binding domain of the Fur regulator on production of pyoverdine

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00426109" target="_blank" >RIV/61388971:_____/13:00426109 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s12223-012-0210-x" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0210-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12223-012-0210-x" target="_blank" >10.1007/s12223-012-0210-x</a>

Result language
angličtina
Original language name
Effect of the amino acid substitution in the DNA-binding domain of the Fur regulator on production of pyoverdine
Original language description
The ferric uptake regulator gene (fur), its promoter region and Fur box of pvdS gene involved in siderophore-mediated iron uptake system were sequenced in the parent strain Pseudomonas aeruginosa PAO1 and in the fur mutant FPA121 derived from the strainPAO1. We identified the gene fur(179) bearing a novel, single-point mutation that changed the amino acid residue Gln60Pro in the DNA-binding domain of the Fur protein. The synthesis of pyoverdine was studied in cultures of the strains PAO1 and FPA121 grown in iron-deplete and iron-replete (60 mu mol/L FeIII) medium. The amino acid replacement in the regulatory Fur protein is responsible for the overproduction of pyoverdine in iron-deplete and iron-replete medium. No mutation was identified in the Fur box of the gene pvdS.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/KJB500200703" target="_blank" >KJB500200703: Characterization of the Fur phenotype of mutants of Pseudomonas aeruginosa: over-production of components of iron transport system.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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