Cardioprotective effect of 2,3-dehydrosilybin preconditioning in isolated rat heart
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73594107" target="_blank" >RIV/61989592:15110/19:73594107 - isvavai.cz</a>
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0367326X18316289?token=CD6DE7C74B94FD82705F4FA9BCD26A12EB71219BE21792C9B4182390E9BE30821F3F2C0332A4CE68D7D551E6072225E3" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0367326X18316289?token=CD6DE7C74B94FD82705F4FA9BCD26A12EB71219BE21792C9B4182390E9BE30821F3F2C0332A4CE68D7D551E6072225E3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fitote.2018.10.028" target="_blank" >10.1016/j.fitote.2018.10.028</a>
Alternative languages
Result language
angličtina
Original language name
Cardioprotective effect of 2,3-dehydrosilybin preconditioning in isolated rat heart
Original language description
2,3-dehydrosilybin (DHS) is a minor component of silymarin, Silybum marianum seed extract, used in some dietary supplements. One of the most promising activities of this compound is its anticancer and cardioprotective activity that results, at least partially, from its cytoprotective, antioxidant, and chemopreventive properties. The present study investigated the cardioprotective effects of DHS in myocardial ischemia and reperfusion injury in rats. Isolated hearts were perfused by the Langendorff technique with low dose DHS (100 nM) prior to 30 min of ischemia induced by coronary artery occlusion. After 60 min of coronary reperfusion infarct size was determined by triphenyltetrazolium staining, while lactatedehydrogenase activity was evaluated in perfusate samples collected at several timepoints during the entire perfusion procedure. Signalosomes were isolated from a heart tissue after reperfusion and involved signalling proteins were detected. DHS reduced the extent of infarction compared with untreated control hearts at low concentration; infarct size as proportion of ischemic risk zone was 7.47 ± 3.1% for DHS versus 75.3 ± 4.8% for ischemia. This protective effect was comparable to infarct limitation induced by ischemic preconditioning (22.3 ± 4.5%). Selective inhibition of Src-family kinases with PP2 (4-Amino-3-(4-chlorophenyl)-1-(t-butyl)-1H-pyrazolo[3,4-d]pyrimidine) abrogated the protection afforded by DHS. This study provides experimental evidence that DHS can mediate Src-kinase-dependent cardioprotection against myocardial damage produced by ischemia/reperfusion injury.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FITOTERAPIA
ISSN
0367-326X
e-ISSN
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Volume of the periodical
132
Issue of the periodical within the volume
January
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
12-21
UT code for WoS article
000456638200003
EID of the result in the Scopus database
2-s2.0-85057143762