All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Metabolite profiling of natural substances in human: in vitro study from fecal bacteria to colon carcinoma cells (Caco-2)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73601530" target="_blank" >RIV/61989592:15110/20:73601530 - isvavai.cz</a>

  • Result on the web

    <a href="https://reader.elsevier.com/reader/sd/pii/S0955286320305143?token=88E9D7AEFA12B178017BB5E2332BD52D9D5BDEBCBD34A7C34841DDEF276D9F0A0227171C6858FF6FEE52EC1C1F503EC1" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0955286320305143?token=88E9D7AEFA12B178017BB5E2332BD52D9D5BDEBCBD34A7C34841DDEF276D9F0A0227171C6858FF6FEE52EC1C1F503EC1</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.jnutbio.2020.108482" target="_blank" >10.1016/j.jnutbio.2020.108482</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Metabolite profiling of natural substances in human: in vitro study from fecal bacteria to colon carcinoma cells (Caco-2)

  • Original language description

    Flavonoids, including anthocyanins, are polyphenolic compounds present in fruits, vegetables and dietary supplements. They can be absorbed from the intestine to the bloodstream or pass into the large intestine. Various bacterial species and enzymes are present along the entire intestine. The aim of the present work was to investigate the intestinal metabolism of selected dietary polyphenol and polyphenol glycosides (quercetin, cyanidin-3-O-glucoside, cyanidin-3-Ogalactoside, and delphinidin-3-O-galactoside) by human fecal bacteria. Moreover, the metabolism of metabolites formed from these compounds in human colon carcinoma cells (Caco-2) was also point of the interest. Test compounds were added to fresh human stool in broth or to Caco-2 cells in medium and then incubated for 6 or 20 h at 37°C. After incubation, samples were prepared for LC/MS determination. Main metabolic pathways were deglycosylation, hydrogenation, methylation, hydroxylation, and decomposition. 2,4,5-trihydroxybenzaldehyde, as a metabolite of cyanidin glycosides, was detected after incubation for the first time. Metabolites formed by fecal bacteria were further glucuronidated or methylated by intestinal enzymes. This metabolite profiling of natural compounds has helped to better understand the complex metabolism in the human intestine and this work also has shown the connection of metabolism of natural substances by intestinal bacteria followed by metabolism in intestinal cells.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30104 - Pharmacology and pharmacy

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    JOURNAL OF NUTRITIONAL BIOCHEMISTRY

  • ISSN

    0955-2863

  • e-ISSN

  • Volume of the periodical

    85

  • Issue of the periodical within the volume

    November 2020

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    11

  • Pages from-to

    "'108482(1)'"-"'108482(11)'"

  • UT code for WoS article

    000582520400018

  • EID of the result in the Scopus database

    2-s2.0-85090982871