Metabolite profiling of natural substances in human: in vitro study from fecal bacteria to colon carcinoma cells (Caco-2)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73601530" target="_blank" >RIV/61989592:15110/20:73601530 - isvavai.cz</a>
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0955286320305143?token=88E9D7AEFA12B178017BB5E2332BD52D9D5BDEBCBD34A7C34841DDEF276D9F0A0227171C6858FF6FEE52EC1C1F503EC1" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0955286320305143?token=88E9D7AEFA12B178017BB5E2332BD52D9D5BDEBCBD34A7C34841DDEF276D9F0A0227171C6858FF6FEE52EC1C1F503EC1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jnutbio.2020.108482" target="_blank" >10.1016/j.jnutbio.2020.108482</a>
Alternative languages
Result language
angličtina
Original language name
Metabolite profiling of natural substances in human: in vitro study from fecal bacteria to colon carcinoma cells (Caco-2)
Original language description
Flavonoids, including anthocyanins, are polyphenolic compounds present in fruits, vegetables and dietary supplements. They can be absorbed from the intestine to the bloodstream or pass into the large intestine. Various bacterial species and enzymes are present along the entire intestine. The aim of the present work was to investigate the intestinal metabolism of selected dietary polyphenol and polyphenol glycosides (quercetin, cyanidin-3-O-glucoside, cyanidin-3-Ogalactoside, and delphinidin-3-O-galactoside) by human fecal bacteria. Moreover, the metabolism of metabolites formed from these compounds in human colon carcinoma cells (Caco-2) was also point of the interest. Test compounds were added to fresh human stool in broth or to Caco-2 cells in medium and then incubated for 6 or 20 h at 37°C. After incubation, samples were prepared for LC/MS determination. Main metabolic pathways were deglycosylation, hydrogenation, methylation, hydroxylation, and decomposition. 2,4,5-trihydroxybenzaldehyde, as a metabolite of cyanidin glycosides, was detected after incubation for the first time. Metabolites formed by fecal bacteria were further glucuronidated or methylated by intestinal enzymes. This metabolite profiling of natural compounds has helped to better understand the complex metabolism in the human intestine and this work also has shown the connection of metabolism of natural substances by intestinal bacteria followed by metabolism in intestinal cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
ISSN
0955-2863
e-ISSN
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Volume of the periodical
85
Issue of the periodical within the volume
November 2020
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
"'108482(1)'"-"'108482(11)'"
UT code for WoS article
000582520400018
EID of the result in the Scopus database
2-s2.0-85090982871