The Effect of Tobacco Smoking and Smoking Cessation on Urinal miRNAs in a Pilot Study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73602127" target="_blank" >RIV/61989592:15110/20:73602127 - isvavai.cz</a>

Alternative codes found

RIV/00098892:_____/20:N0000194

Result on the web

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554876/pdf/life-10-00191.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7554876/pdf/life-10-00191.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/life10090191" target="_blank" >10.3390/life10090191</a>

Result language

angličtina

Original language name

The Effect of Tobacco Smoking and Smoking Cessation on Urinal miRNAs in a Pilot Study

Original language description

The diseases associated with tobacco smoking affect miRNAs and small single-stranded non-coding RNAs. However, there are no data on urinal miRNAs in healthy smokers. We searched for the possible effect of smoking and smoking cessation on miRNA urine expression. For screening, Affymetrix miRNA 4.0 arrays were used in 33 urine samples obtained from six never smokers and from current smokers in three time-points before smoking cessation (n= 10), after short time abstinence (3-8 weeks), and after long-term abstinence (1 year). For validation, a quantitative (q) polymerase chain reaction (PCR) method was used in 93 urine samples obtained from 18 never smokers and 25 current smokers in three time-points before smoking cessation, after short time abstinence (3-8 weeks), and after long-term abstinence (1 year). In screening analysis, 5 miRNAs (hsa-miR-3620-5p, hsa-miR-3613-5p, hsa-miR-3921, hsa-miR-5094, and hsa-miR-337-3p) were dysregulated in current vs. never smokers after multiple testing corrections. Smoking cessation was accompanied by miRNA dysregulation that did not reach a significant level after a multiple testing correction. In validation analysis, three miRNAs correlated with cotinine, but they were affected neither after smoking cessation nor between current and never smokers. Our whole-genome screening of 2.578 miRNAs and validation suggest that tobacco smoking has no or only a small effect on urinal miRNAs.

Czech name

—

Czech description

—

Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

—

OECD FORD branch

10606 - Microbiology

Project

<a href="/en/project/EF16_019%2F0000868" target="_blank" >EF16_019/0000868: Molecular, cellular and clinical approach to healthy ageing</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Life

ISSN

2075-1729

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

9

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

"'191(1)'"-"'191(13)'"

UT code for WoS article

000580094500001

EID of the result in the Scopus database

2-s2.0-85090649763