Ion-exchange HPLC-ICP-MS: A new window to chromium speciation in biological tissues
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73603935" target="_blank" >RIV/61989592:15110/20:73603935 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/20:73603935
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0039914020304410" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914020304410</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.talanta.2020.121150" target="_blank" >10.1016/j.talanta.2020.121150</a>
Alternative languages
Result language
angličtina
Original language name
Ion-exchange HPLC-ICP-MS: A new window to chromium speciation in biological tissues
Original language description
The presented work proposes a novel analytical ICP-MS-based approach for the accurate and precise chromium speciation in biological tissues. The determination of total Cr(VI) and soluble Cr(III) species was carried out by alkaline EDTA extraction followed by their separation using ion-exchange high-performance liquid chromatography inductively coupled plasma mass spectrometry (IE-HPLC-ICP-MS). The developed method was validated according to the procedure given in the United States Food and Drug Administration guideline on the validation of bioanalytical methods. Validation parameters included limit of detection (<= 0.03 mu g g(-1)), limit of quantification (<= 0.08 mu g g(-1)), linearity (>= 0.9998), intra-day and inter-day accuracy (86-110%) and precision ( 10%), extraction recovery (89-110%), carry-over effect and sensitivity. In addition, special attention was paid to the study of chromium species interconversion and the elimination of spectral interferences. Moreover, the validated ICP-MS method employing microwave acid digestion was used to determine the total Cr content in collected fractions. Finally, the whole ICP-MS-based methodology was applied to the analyses of two certified reference materials of hepatopancreas tissue. Obtained results indicated that the majority of chromium in biological tissues is bound to the solid residue, Cr(VI) was determined in none of the samples investigated. This is the first study focusing on soluble Cr(III), total Cr(VI), and total bound Cr species in biological tissues. It is characterized by efficient sample preparation and fast simultaneous analysis of Cr species with parallel total Cr analysis serving for chromium balance evaluation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
TALANTA
ISSN
0039-9140
e-ISSN
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Volume of the periodical
218
Issue of the periodical within the volume
OCT
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
"121150-1"-"121150-8"
UT code for WoS article
000564732500006
EID of the result in the Scopus database
2-s2.0-85085150826