Study of chromium species release from metal implants in blood and joint effusion: Utilization of HPLC-ICP-MS

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73591359" target="_blank" >RIV/61989592:15110/18:73591359 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/18:73591359

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0039914018303424" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0039914018303424</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2018.03.100" target="_blank" >10.1016/j.talanta.2018.03.100</a>

Result language

angličtina

Original language name

Study of chromium species release from metal implants in blood and joint effusion: Utilization of HPLC-ICP-MS

Original language description

The objective of this study was to develop and validate a novel analytical procedure for determination of total chromium and Cr(III) and Cr(VI) species released from metal implants into whole blood and joint effusion. Firstly, the ion-pair chromatographic method employing reversed-phase high-performance liquid chromatography inductively coupled plasma mass spectrometry (ICP-MS) for analysis of species was developed. Secondly, all samples, protein and low molecular fractions were analyzed for their total chromium content using ICP-MS. This new measurement procedure was validated by the following parameters: limit of detection (0.13 mu g L-1 for Cr(III), 0.14 mu g L-1 for Cr(VI)), linearity of calibration, trueness (recovery 84-92%), intermediate precision (RSD &lt; 5%). We determined statistically significantly higher chromium levels in joint effusion samples obtained from patients in comparison with a control group. On the other hand, no relevant difference among the concentrations of both species and total chromium in blood was observed. Our results show that the majority of chromium is present in the trivalent form and bound to proteins. This speciation study is rare in the field of speciation analysis in clinical samples. It is characterized by very fast and simple sample preparation without any changes in distribution or stability of both Cr forms and efficient simultaneous analysis of Cr species.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

TALANTA

ISSN

0039-9140

e-ISSN

—

Volume of the periodical

185

Issue of the periodical within the volume

AUG

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

370-377

UT code for WoS article

000433656200050

EID of the result in the Scopus database

2-s2.0-85044963646