Strengths and Weaknesses of Cell Synchronization Protocols Based on Inhibition of DNA Synthesis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F21%3A73607792" target="_blank" >RIV/61989592:15110/21:73607792 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/19/10759/htm" target="_blank" >https://www.mdpi.com/1422-0067/22/19/10759/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms221910759" target="_blank" >10.3390/ijms221910759</a>
Alternative languages
Result language
angličtina
Original language name
Strengths and Weaknesses of Cell Synchronization Protocols Based on Inhibition of DNA Synthesis
Original language description
Synchronous cell populations are commonly used for the analysis of various aspects of cellular metabolism at specific stages of the cell cycle. Cell synchronization at a chosen cell cycle stage is most frequently achieved by inhibition of specific metabolic pathway(s). In this respect, various protocols have been developed to synchronize cells in particular cell cycle stages. In this review, we provide an overview of the protocols for cell synchronization of mammalian cells based on the inhibition of synthesis of DNA building blocks—deoxynucleotides and/or inhibition of DNA synthesis. The mechanism of action, examples of their use, and advantages and disadvantages are described with the aim of providing a guide for the selection of suitable protocol for different studied situations.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN
1422-0067
e-ISSN
—
Volume of the periodical
2021
Issue of the periodical within the volume
22
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
"nestránkováno"
UT code for WoS article
000707134200001
EID of the result in the Scopus database
2-s2.0-85116220991