Soluble epoxide hydrolase as an important player in intestinal cell differentiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F21%3A73611535" target="_blank" >RIV/61989592:15110/21:73611535 - isvavai.cz</a>

Result on the web

<a href="https://obd.upol.cz/id_publ/333191422" target="_blank" >https://obd.upol.cz/id_publ/333191422</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000512807" target="_blank" >10.1159/000512807</a>

Result language

angličtina

Original language name

Soluble epoxide hydrolase as an important player in intestinal cell differentiation

Original language description

There is a growing evidence that soluble epoxide hydrolase (sEH) may play a role in cell differentiation. sEH metabolizes biologically highly active and generally cytoprotective epoxyeicosatrienoic acids (EETs), generated from arachidonic acid metabolism by CYP epoxygenases (CYP2C and CYP2J subfamilies), to less active corresponding diols. We investigated the effect of sEH inhibitor (TPPU) on expression of villin, CYP2C8, CYP2C9, CYP2J2 and sEH in undifferentiated and in vitro differentiated HT-29 and Caco2 cell lines. The administration of 10 μM TPPU on differentiated HT-29 and Caco2 cells resulted in a significant decrease in expression of villin, marker of intestinal cell differentiation. It was accompanied by disruption of brush border when microvilli appeared sparse and short in atomic force microscope scans of HT-29 cells. Although inhibition of sEH in differentiated HT-29 and Caco2 cells led to increase in sEH expression in both cell lines, this treatment had an opposite effect on CYP2J2 expression in HT-29 and Caco2. In addition, tissue samples of colorectal carcinoma and adjacent normal tissues from 45 patients were immunostained for sEH and villin. We detected a significant decrease in expression of both proteins in colorectal carcinoma in comparison to adjacent normal tissue, and the decrease in both sEH and villin expression revealed moderate positive association. Taken together, our results proved that sEH is an important player in intestinal cell differentiation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CELLS TISSUES ORGANS

ISSN

1422-6405

e-ISSN

—

Volume of the periodical

209

Issue of the periodical within the volume

4-6

Country of publishing house

CH - SWITZERLAND

Number of pages

12

Pages from-to

177-188

UT code for WoS article

000619097700001

EID of the result in the Scopus database

2-s2.0-85100902457