When activator and inhibitor of PPARα do the same: Consequence for differentiation of human intestinal cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F21%3A73611451" target="_blank" >RIV/61989592:15110/21:73611451 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2227-9059/9/9/1255/htm" target="_blank" >https://www.mdpi.com/2227-9059/9/9/1255/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/biomedicines9091255" target="_blank" >10.3390/biomedicines9091255</a>
Alternative languages
Result language
angličtina
Original language name
When activator and inhibitor of PPARα do the same: Consequence for differentiation of human intestinal cells
Original language description
Peroxisome proliferator-activated receptor α (PPARα) is a ligand-dependent transcription factor which play a role in various processes, including differentiation of several cell types. We investigated the role of PPARα in differentiation of intestinal cells using HT-29 cell line as a model as well as human normal colon and colorectal carcinoma tissues. We detected significant increase in PPARα expression in differentiated HT-29 cells as well as in normal surface colon epithelium where differentiated cells are localized. Thus, it seemed that PPARα may play a role in differentiation of intestinal cells. Interestingly, we found that both, PPARα activator fenofibrate as well as its inhibitor GW6471 regulated proliferation and differentiation of HT-29 cells in vitro in the same way. Both compounds led to decrease in proliferation accompanied by significant increase in expression of villin, intestinal alkaline phosphatase (differentiation markers) together with increase in lipid droplets accumulation. Furthermore, villin expression was independent on subcellular localization of PPARα. Moreover, we found similar levels of PPARα expression in colorectal carcinomas in comparison to adjacent normal epithelium. All these finding support the hypothesis, that differentiation of intestinal epithelium is PPARα-independent.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomedicines
ISSN
2227-9059
e-ISSN
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Volume of the periodical
2021
Issue of the periodical within the volume
9
Country of publishing house
CH - SWITZERLAND
Number of pages
17
Pages from-to
"nestránkováno"
UT code for WoS article
000699234400001
EID of the result in the Scopus database
2-s2.0-85115798949