Detection of clinically important β-lactamases by using PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F21%3A73612165" target="_blank" >RIV/61989592:15110/21:73612165 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/femsle/article/368/11/fnab068/6294906?login=true" target="_blank" >https://academic.oup.com/femsle/article/368/11/fnab068/6294906?login=true</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/femsle/fnab068" target="_blank" >10.1093/femsle/fnab068</a>
Alternative languages
Result language
angličtina
Original language name
Detection of clinically important β-lactamases by using PCR
Original language description
Increasing antimicrobial resistance of nosocomial pathogens is becoming a serious threat to public health. To control the spread of this resistance, it is necessary to detect β-lactamase-producing organisms in the clinical setting. The aims of the study were to design a PCR assay for rapid detection of clinically encountered β-lactamase genes described in Enterobacteriaceae and Gram-negative non-fermenting bacteria. The functionality of proposed primers was verified using eight reference strains and 17 strains from our collection, which contained 29 different β-lactamase genes. PCR products of the test strains were confirmed by Sanger sequencing. Sequence analysis was performed using bioinformatics software Geneious. Overall, 67 pairs of primers for detecting 12 members of the class C β-lactamase family, 15 members of class A β-lactamases, six gene families of subclass B1, one member each of subclasses B2, B3 and class D β-lactamases were designed, of which 43 pairs were experimentally tested in vitro. All 29 β-lactamase genes, including 10 oxacillinase subgroups, were correctly identified by PCR. The proposed set of primers should be able to specifically detect 99.7% of analyzed β-lactamase subtypes and more than 79.8% of all described β-lactamase genes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS MICROBIOLOGY LETTERS
ISSN
0378-1097
e-ISSN
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Volume of the periodical
368
Issue of the periodical within the volume
11
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
"nestránkováno"
UT code for WoS article
000670887100004
EID of the result in the Scopus database
2-s2.0-85108386470