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Detection of clinically important β-lactamases by using PCR

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F21%3A73612165" target="_blank" >RIV/61989592:15110/21:73612165 - isvavai.cz</a>

  • Result on the web

    <a href="https://academic.oup.com/femsle/article/368/11/fnab068/6294906?login=true" target="_blank" >https://academic.oup.com/femsle/article/368/11/fnab068/6294906?login=true</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/femsle/fnab068" target="_blank" >10.1093/femsle/fnab068</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Detection of clinically important β-lactamases by using PCR

  • Original language description

    Increasing antimicrobial resistance of nosocomial pathogens is becoming a serious threat to public health. To control the spread of this resistance, it is necessary to detect β-lactamase-producing organisms in the clinical setting. The aims of the study were to design a PCR assay for rapid detection of clinically encountered β-lactamase genes described in Enterobacteriaceae and Gram-negative non-fermenting bacteria. The functionality of proposed primers was verified using eight reference strains and 17 strains from our collection, which contained 29 different β-lactamase genes. PCR products of the test strains were confirmed by Sanger sequencing. Sequence analysis was performed using bioinformatics software Geneious. Overall, 67 pairs of primers for detecting 12 members of the class C β-lactamase family, 15 members of class A β-lactamases, six gene families of subclass B1, one member each of subclasses B2, B3 and class D β-lactamases were designed, of which 43 pairs were experimentally tested in vitro. All 29 β-lactamase genes, including 10 oxacillinase subgroups, were correctly identified by PCR. The proposed set of primers should be able to specifically detect 99.7% of analyzed β-lactamase subtypes and more than 79.8% of all described β-lactamase genes.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FEMS MICROBIOLOGY LETTERS

  • ISSN

    0378-1097

  • e-ISSN

  • Volume of the periodical

    368

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

    "nestránkováno"

  • UT code for WoS article

    000670887100004

  • EID of the result in the Scopus database

    2-s2.0-85108386470